货号： CL-0118
Price： ￥1600 ￥1600
生长培养基： McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | HT-29（人结肠癌细胞） |
| 别称 | HT 29; HT29 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的；近来，已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
| 年龄（性别） | 44Y Female |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
| 受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
STR位点信息
STR鉴定图
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Cucurbitacin B induces apoptosis in colorectal cells through reactive oxygen species generation and endoplasmic reticulum stress pathways(2023)
作者：Jian-Lan Huang, Li Liang, Pei-En Xie, Wei-Liang Sun, Li Wang, Zheng-Wen Cai
期刊：Experimental and Therapeutic Medicine
影响因子：2.7
引用产品： HT-29 细胞, SW620 细胞
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Culturable Diversity and Biological Properties of Bacterial Endophytes Associated with the Medicinal Plants of Vernonia anthelmintica (L.) Willd(2023)
作者：Niu Litao, Nigora Rustamova, Paiziliya Paerhati, Hui-Xia Ning, Abulimiti Yili
期刊：Applied Sciences-Basel
影响因子：2.7
引用产品： HT-29 细胞
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Let-7c-5p Down Regulates the Proliferation of Colorectal Cancer Through the MAPK-ERK-Signaling Pathway(2023)
作者：Fu Juanjuan, Zhou Longmei, Li Sijin, He Wenjing, Zheng Jining, Hou Zhiping, He Peiyuan
期刊：BIOCHEMICAL GENETICS
DOI：10.1007/s10528-023-10581-9
影响因子：2.4
引用产品： HCT-8 [HRT-18] 细胞, HT-29 细胞, DMEM高糖 培养基, 特级胎牛血清
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NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer(2023)
作者：Hong Yang, Leilei Pu, Ruobing Li, Rong Zhu
期刊：Journal of Gastrointestinal Oncology
影响因子：2.1
引用产品： HCT 116 细胞, SW480 [SW-480] 细胞, HT-29 细胞
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Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer(2022)
作者：Meng Tang, Hui Xu, Hongyan Huang, Hao Kuang, Chenxi Wang, Qinqin Li, Xin Zhang, Yizhong Ge, Mengmeng Song, Xi Zhang, Ziwen Wang, Chaobing Ma, Jinlin Kang, Wanfang Zhang, You Wang, Bo Zhang, Xiaowei Zhang, Yongbing Chen, Minghua Cong, Gerry Melino, Xiaobin Wang, Fuxiang Zhou, Qiang Sun, Hanping Shi
期刊：Advanced Science
影响因子：17.5
引用产品： SW480 [SW-480] 细胞, HT-29 细胞, SW620 细胞
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Nitroreductase-instructed supramolecular assemblies for microbiome regulation to enhance colorectal cancer treatments(2022)
作者：Jiali Chen, Pai Zhang, Yan Zhao, Jie Zhao, Xiaobo Wu, Ruijia Zhang, Ruitao Cha, Qingxin Yao, Yuan Gao
期刊：Science Advances
影响因子：15.0
引用产品： Hela 细胞, HT-29 细胞, HCT 116 细胞, CT26.WT 细胞, DMEM高糖 培养基, McCoy's 5A 培养基, RPMI-1640 培养基
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022)
作者：Jin Chi, Wang Tuo, Zhang Dongsheng, Yang Peng, Zhang Chuan, Peng Wen, Jin Kangpeng, Wang Lu, Zhou Jiahui, Peng Chaofan, Tan Yuqian, Ji Jiangzhou, Chen Zhihao, Sun Qingyang, Yang Sheng, Tang Junwei, Feng Yifei, Sun Yueming
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影响因子：12.7
引用产品： 293T [HEK-293T] 细胞, NCM460 细胞, SW480 [SW-480] 细胞, DLD-1 细胞, HT-29 细胞, RKO 细胞, HCT 116 细胞
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Anti-colon cancer effects of Spirulina polysaccharide and its mechanism based on 3D models(2022)
作者：Yingfang Lu, Zexin Chen, Qianmin Lin, Xuewei Xia, Yuqi Lin, Jun Yan, Min Huang, Riming Huang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2022.12.244
影响因子：8.0
引用产品： LoVo 细胞, HT-29 细胞, HCT 116 细胞
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Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest(2022)
作者：Cheng Junwen, Wang Yanbin, Song Jiling, Liu Yu, Ji Weiwei, He Liang, Wei Hailong, Hu Chuanjiu, Jiang Yihan, Xing Yiqi, Huang Xubo, Ding Hongmei, He Qinghai
期刊：Frontiers in Nutrition
影响因子：6.6
引用产品： HT-29 细胞
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SEDDS facilitate cinnamaldehyde crossing the mucus barrier: The perspective of mucus and Caco-2/HT29 co-culture models(2022)
作者：Ye Cai, Liu Liu, Mengqiu Xia
期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2022.121461
影响因子：5.8
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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