货号： CL-0118
Price： ￥1600 ￥1600
生长培养基： McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
产品信息
| 产品名称 | HT-29（人结肠癌细胞） |
| 别称 | HT 29; HT29 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120] 培养条件: 气相：空气，95%；CO₂，5%, 温度：37℃ |
| 培养方案B（可选） |
生长培养基: 培养条件: 气相：, 温度：37℃ |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的；近来，已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
| 年龄（性别） | 44Y Female |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
| 受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
STR位点信息
STR鉴定图
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A possible but unrecognized risk of acceptable daily intake dose triazole pesticides exposure—bile acid disturbance induced pharmacokinetic changes of oral medication(2023)
作者：Yujing Xu, Yufeng Zhang, Quan Tao, Quanwei Sun, Yuyu Zheng, Dengke Yin, Ye Yang
期刊：CHEMOSPHERE
DOI：10.1016/j.chemosphere.2023.138209
影响因子：8.8
引用产品： HT-29 细胞, McCoy's 5A 培养基
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USP10 strikes down β-catenin by dual-wielding deubiquitinase activity and phase separation potential(2023)
作者：Yinuo Wang, Aihua Mao, Jingwei Liu, Pengjie Li, Shaoqin Zheng, Tong Tong, Zexu Li, Haijiao Zhang, Lanjing Ma, Jiahui Lin, Zhongqiu Pang, Qing Han, Fukang Qi, Xinjun Zhang, Maorong Chen, Xi He, Xi Zhang, Teng Fei, Bi-Feng Liu, Daming Gao, Liu Cao, Qiang Wang, Yiwei Li, Ren Sheng
期刊：Cell Chemical Biology
DOI：10.1016/j.chembiol.2023.07.016
影响因子：8.6
引用产品： HT-29 细胞
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Targeted EpCAM-binding for the development of potent and effective anticancer proteins(2023)
作者：Zhao Liu, Chen Zhang, Beiming Cui, Yijie Wang, Kaisheng Lim, Kai Li, Jean Paul Thiery, Jun Chen, Chun Loong Ho
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114443
影响因子：7.5
引用产品： HT-29 细胞, MCF7 [MCF-7] 细胞, Hela 细胞, Caco-2 细胞
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Side population cells derived from hUCMSCs and hPMSCs could inhibit the malignant behaviors of Tn+ colorectal cancer cells from modifying their O-glycosylation status(2023)
作者：Hu Wen, Ding Ruisong, Wang Mengyang, Huang Panpan, Wei Xia, Hu Xingyou, Hu Tao
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-023-03334-3
影响因子：7.5
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Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics(2023)
作者：Tingting Li, Hang Li, Wenxin Xia, Mengyuan Li, Yang Niu, Xueyan Fu
期刊：Oxidative Medicine and Cellular Longevity
影响因子：7.3
引用产品： HT-29 细胞, HCT 116 细胞
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3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis(2023)
作者：Mu Mingchao, Zhang Qin, Zhao Chenye, Li Xiaopeng, Chen Zilu, Sun Xuejun, Yu Junhui
期刊：CANCER GENE THERAPY
DOI：10.1038/s41417-023-00648-5
影响因子：6.4
引用产品： HT-29 细胞
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USP7-mediated JUND suppresses RCAN2 transcription and elevates NFATC1 to enhance stem cell property in colorectal cancer(2023)
作者：Chang Yunli, Chen Lingling, Tang Jie, Chen Guoyu, Ji Jieru, Xu Ming
期刊：CELL BIOLOGY AND TOXICOLOGY
DOI：10.1007/s10565-023-09822-9
影响因子：6.1
引用产品： HT-29 细胞, HCT 116 细胞, LoVo 细胞, CW-2 细胞
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer(2023)
作者：Di Ziyang, Xu Gaoran, Ding Zheyu, Li Chengxin, Song Jialin, Huang Guoquan, Zheng Jinsen, Zhang Xinyao, Xiong Bin
期刊：Cancer Cell International
DOI：10.1186/s12935-023-03025-2
影响因子：5.8
引用产品： NCM460 细胞, SW620 细胞, HCT 116 细胞, SW480 [SW-480] 细胞, DLD-1 细胞, LoVo 细胞, HT-29 细胞
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A Genetic Algorithm-Based Ensemble Learning Framework for Drug Combination Prediction(2023)
作者：Lianlian Wu, Xiaona Ye, Yixin Zhang, Jie Gao, Zhikai Lin, Binsheng Sui, Yuqi Wen, Qingqiang Wu, Kunhong Liu, Song He, Xiaochen Bo
期刊：Journal of Chemical Information and Modeling
影响因子：5.6
引用产品： HT-29 细胞
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Gentianine alleviates dextran sulfate sodium-induced ulcerative colitis via inhibition of TLR4/NLRP3-mediated pyroptosis(2023)
作者：Yun-xia Li, Lei Lv, Shao-liang Li, Hai-hua Qian
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.111214
影响因子：5.6
引用产品： HT-29 细胞, McCoy's 5A 培养基
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Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？
部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养条件，出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件，为了避免细胞突然更换培养条件后不适应，建议您优先使用厂家推荐的培养条件培养。
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