HT-29(人结肠癌细胞)

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货号: CL-0118

Price: ¥1600 ¥1600

Size:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

生长培养基: McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]

产品信息

产品名称 HT-29(人结肠癌细胞)
别称 HT 29; HT29
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:McCoy's 5A[PM150710]+10% FBS[164210]+1% P/S[PB180120]
培养条件: 气相:空气,95%;CO₂,5%, 温度:37℃
培养方案B(可选) 生长培养基:
培养条件: 气相:, 温度:37℃
推荐传代比例 1:3-1:6
推荐换液频率 2-3次/周

参考资料(来源文献)

背景描述 HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的;近来,已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。
年龄(性别) 44Y Female
组织来源 结直肠腺癌
细胞类型 肿瘤细胞
肿瘤类型 肠癌细胞
生物安全等级 BSL-1
致瘤性 Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters.
受体表达情况 human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A

STR位点信息

STR鉴定图

  • Solid self-microemulsifying drug delivery system (S-SMEDDS) prepared by spray drying to improve the oral bioavailability of cinnamaldehyde (CA)(2024)

    作者:Yun Meng, Ye Cai, Mengyao Cui, Yuhang Xu, Long Wu, Xiang Li, Xiaoqin Chu

    期刊:PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY

    DOI:10.1080/10837450.2024.2312851

    影响因子:2.6

    引用产品: Caco-2 细胞, HT-29 细胞

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    期刊:DIGESTIVE DISEASES AND SCIENCES

    DOI:10.1007/s10620-024-08735-9

    影响因子:2.5

    引用产品: HCT-15 细胞, HCT 116 细胞, HT-29 细胞, SW480 [SW-480] 细胞

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    期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

    DOI:10.1016/j.bbrc.2024.150262

    影响因子:2.5

    引用产品: HT-29 细胞, SW480 [SW-480] 细胞

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    作者:Bingbing Shang, Haiyan Qiao, Liang Wang, Jingyu Wang

    期刊:PeerJ

    DOI:10.7717/peerj.18374

    影响因子:2.3

    引用产品: HCT 116 细胞, SW480 [SW-480] 细胞, HT-29 细胞, SW620 细胞

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    期刊:PeerJ

    DOI:10.7717/peerj.18406

    影响因子:2.3

    引用产品: HT-29 细胞, SW480 [SW-480] 细胞, HCT 116 细胞, SW620 细胞

  • EⅡB Mutation Reduces the Pathogenicity of Listeria monocytogenes by Negatively Regulating Biofilm Formation Ability, Infective Capacity, and Virulence Gene Expression(2024)

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    期刊:Veterinary Sciences

    DOI:10.3390/vetsci11070301

    影响因子:2.0

    引用产品: HT-29 细胞, RAW 264.7 细胞, MEM 培养基

  • The role of chromatin modulator DPY30 in glucose metabolism of colorectal cancer cells(2024)

    作者:Xiaomei Mao, Shiqin Yang, Ye Zhang, Huajun Yang, Danhong Yan, Lingzhi Zhang

    期刊:Translational Cancer Research

    DOI:10.21037/tcr-24-366

    影响因子:1.5

    引用产品: KM12C 细胞, HT-29 细胞

  • JAML inhibits colorectal carcinogenesis by modulating the tumor immune microenvironment(2024)

    作者:Cheng Shiliang, Li Meng, Li Chunguang, Dai Yonggang, Zhuo Jinhua, Wang Jue, Qian Jingrong, Hao Zhihao

    期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

    DOI:10.1007/s11626-024-00881-8

    影响因子:1.5

    引用产品: HT-29 细胞, SW620 细胞

  • IL6ST: A Novel Therapeutic Target for Managing and Treating Colorectal Cancer Via Ferroptosis(2024)

    作者:Kun Zhao, Baoguo He, Kuijin Xue, Bin Cao, Keyu Ren, Yanchun Jin, Shanwei Rong, Liangzhou Wei, Hongyun Wei

    期刊:Turkish Journal of Gastroenterology

    DOI:10.5152/tjg.2024.23353

    影响因子:1.4

    引用产品: NCM460 细胞, HCT 116 细胞, HT-29 细胞, SW620 细胞

  • Bacteria-Induced Colloidal Encapsulation for Probiotic Oral Delivery(2023)

    作者:Chong Zhang, Xiaorong Gao, Xinxiu Ren, Ting Xu, Qiang Peng, Yixin Zhang, Zhenhua Chao, Wenning Jiang, Lingyun Jia, Lulu Han

    期刊:ACS Nano

    DOI:10.1021/acsnano.3c00600

    影响因子:17.1

    引用产品: HT-29 细胞, Caco-2 细胞

  • Q1:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?

    部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。

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