产品概述
产品信息
| 名称 | U-2932 (人弥漫大B淋巴瘤细胞) (STR鉴定正确) |
| 别称 | U2932 |
| 种属 | 人 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样，圆形，单个细胞，少数聚团 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640＋10% FBS＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging |
| 年龄（性别） | 女性；29岁 |
| 组织来源 | 腹水 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 48 hours (PubMed=12533045); ~50 hours (DSMZ=ACC-633) |
| 保藏机构 | DSMZ; ACC-633 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 22,23 D3S1358 15,16 D5S818 12 D7S820 9,13 D8S1179 13,14 D13S317 11 D16S539 12 D18S51 15 D19S433 14 D21S11 28,30 FGA 22,24 PentaD 14 PentaE 13,14 TH01 9.3 TPOX 11,12 vWA 14,17 D6S1043 18 D12S391 18,20 D2S441 12,15 -
STR鉴定图
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