产品概述
产品信息
| 名称 | THLE-2 (人肝永生化细胞) (STR鉴定正确) |
| 别称 | THLE2; Transformed Human Liver Epithelial-2 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55%DM/F12+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
THLE-2 专用培养基
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways. |
| 年龄（性别） | 男性 |
| 组织来源 | 肝脏；左叶 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 致瘤性 | No; No, nude mice |
| 保藏机构 | ATCC; CRL-2706 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 11,13 D2S1338 17,23 D3S1358 14,15 D5S818 11,13 D7S820 10,12 D8S1179 13 D13S317 8,12 D16S539 11,13 D18S51 13,16 D19S433 14 D21S11 30,32.2 FGA 22,24 PentaD 10,13 PentaE 12,13 TH01 7,9.3 TPOX 8,11 vWA 16,17 D6S1043 12,18 D12S391 21,23 D2S441 11 -
STR鉴定图
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