产品概述
名称 | SU-DHL-2 (人弥散性组织淋巴瘤细胞) (STR鉴定正确) |
别称 | SUDHL2; SUDHL-2; Su-DHL-2; SU-DHL2; SuDHL 2; Stanford University-Diffuse Histiocytic Lymphoma-2; DHL-2 |
种属 | 人类 |
生长特性 | 悬浮细胞,多细胞聚集 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
RPMI-1640+10% FBS+1% P/S
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3次/周 |
背景描述 | SU-DHL-2 is a histiocytic cell line that was isolated in 1974 from the lymph node of a White 73-year-old female with large cell lymphoma. The cells are non-specific esterase and acid phosphatase positive. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). The cells are surface Ig negative (sIG-). The cells are reported to be E - rosette negative. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR. |
年龄(性别) | 女性;73岁 |
组织来源 | 淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 大细胞淋巴瘤 |
生物安全等级 | BSL-1 |
倍增时间 | ~24 hours |
保藏机构 | ATCC; CRL-2956 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,24 D3S1358 14,19 D5S818 13 D7S820 11,13 D8S1179 13 D13S317 11,12 D16S539 14 D18S51 11 D19S433 12,15.2 D21S11 30.2 FGA 21,25 PentaD 13 PentaE 7,11 TH01 6,9 TPOX 8 vWA 17 D6S1043 17 D12S391 16,23 D2S441 11,13 -
STR鉴定图
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