产品概述
产品信息
| 名称 | SK-N-DZ (人神经母细胞瘤细胞) (STR鉴定正确) |
| 别称 | SKNDZ |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成神经细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
DMEM＋10% FBS＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理；细胞离心复苏之后有少量漂浮，第二天换液即可，每三天换液一次，等细胞密度达到80%左右，按照1:2进行传代，传代之后有少量漂浮，换液即可，消化时间在显微镜下观察，以细胞变圆，脱落为主，大约控制消化时间在4-6mim左右。 |
参考资料(来源文献)
| 背景描述 | SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| 年龄（性别） | 女性；2岁 |
| 组织来源 | 大脑 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 神经母细胞瘤细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes; Yes, forms tumors in nude mice |
| 保藏机构 | ATCC; CRL-2149 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 21,22,23 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11 D16S539 9,11 D18S51 15,17 D19S433 14.2,16 D21S11 29,31 FGA 18,22 PentaD 9,14 PentaE 7,10 TH01 6,9 TPOX 8 vWA 16,18 D6S1043 14,19 D12S391 18,21 D2S441 10,11 -
STR鉴定图
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