产品概述
产品信息
| 名称 | Panc 08.13 (人胰腺癌细胞) (STR鉴定正确) |
| 别称 | Panc 8.13; Panc-08.13; PANC-08-13; Panc_08_13; Panc08.13; Panc8.13; Panc-8_13; Panc-813; PANC 813; Panc813; PANC813; PANC0813; Panc0813; Pa14C; PL9; PL-9 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640＋15% FBS＋10μg/mL Insulin ＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | Panc 08.13 is an epithelial cell line that was isolated in 1995 from the pancreas of a White, 85-year-old, male patient with adenocarcinoma. This cell line was deposited by EM Jaffee and can be used in cancer research.The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%. |
| 年龄（性别） | 男性；85岁 |
| 组织来源 | 胰腺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 胰腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 20.0 hours (PubMed=9612602); 117 hours (PubMed=25984343) |
| 致瘤性 | Yes; Yes, forms tumors in nude or SCID mice |
| 抗原表达情况 | MHC class I +; MHC class II - |
| 基因表达情况 | cytokeratin 7; cytokeratin 18 |
| 保藏机构 | ATCC; CRL-2551 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 17,18 D3S1358 16 D5S818 13,14 D7S820 11 D8S1179 10,14 D13S317 13 D16S539 13 D18S51 19 D19S433 13 D21S11 32.2 FGA 21,23 PentaD 10 PentaE 16,17 TH01 6,9.3 TPOX 8,12 vWA 18 D6S1043 11,14 D12S391 23 D2S441 10,11.3 -
STR鉴定图
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武汉市高新大道858号光谷生物医药产业园二期B18栋
