产品概述
产品信息
| 名称 | NCI-H526 (人小细胞肺癌细胞) (STR鉴定正确) |
| 别称 | H526; H-526; NCIH526 |
| 种属 | 人 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 圆形聚团 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640+10%FBS+1%P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | NCI-H526 [H526] is an epithelial cell line that was isolated from the lungs of a 55-year-old, White male with carcinoma.NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed. NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected. RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. Abnormally sized mRNA was present. |
| 年龄（性别） | 男性；55岁 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 小细胞肺癌 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 36-48 hours |
| 致瘤性 | Yes; Yes, the cells produce tumors in athymic nude mice |
| 保藏机构 | ATCC; CRL-5811 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 23,24 D3S1358 17 D5S818 11 D7S820 9,12 D8S1179 14 D13S317 13 D16S539 9 D18S51 12,15 D19S433 13,15 D21S11 27,32.2 FGA 22 PentaD 13,14 PentaE 5,13 TH01 6,8 TPOX 8,11 vWA 16,17 D6S1043 11 D12S391 15,18 D2S441 11,14 -
STR鉴定图
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武汉市高新大道858号光谷生物医药产业园二期B18栋
