产品概述
产品信息
| 名称 | HS-27A (人骨髓细胞) (STR鉴定正确) |
| 别称 | HS27A; HS27a |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640+10%FBS+1%P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1). |
| 年龄（性别） | 男性；30岁 |
| 组织来源 | 骨头；骨髓；基质 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~36h-48 hours |
| 抗原表达情况 | Vascular cell adhesion molecule 1 (CD106, VCAM-1) |
| 应用领域 | 3D cell culture； Immunology |
| 保藏机构 | ATCC; CRL-2496 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 10,11 D2S1338 22 D3S1358 14,17 D5S818 12 D7S820 12 D8S1179 13,16 D13S317 11,13 D16S539 11,12 D18S51 12,15 D19S433 15 D21S11 29,30 FGA 21,24 PentaD 10,13 PentaE 5,6 TH01 7,9 TPOX 8,11 vWA 18,19 D6S1043 15,18 D12S391 19,21 D2S441 11 -
STR鉴定图
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武汉市高新大道858号光谷生物医药产业园二期B18栋
