产品概述
产品信息
| 名称 | DoHH2 (人B细胞淋巴瘤细胞) (STR鉴定正确) |
| 别称 | DOHH2; DoHH-2; DOHH-2 |
| 种属 | 人源 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640＋10% FBS＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described in the literature to carry the t(14;18) leading to IGH-BCL2 fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| 年龄（性别） | 男性；60岁 |
| 组织来源 | 胸腔积液 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~40 hours |
| 保藏机构 | DSMZ; ACC-47 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 11,12 D2S1338 22,24 D3S1358 15,17 D5S818 11,12 D7S820 11 D8S1179 13,16 D13S317 12,13 D16S539 11,14 D18S51 11,14 D19S433 14.2,15.2 D21S11 30 FGA 21,27 PentaD 9,12 PentaE 12,16 TH01 9 TPOX 8,11 vWA 14,19 D6S1043 11,12 D12S391 19,20 D2S441 11,14 -
STR鉴定图
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