产品概述
产品信息
| 名称 | DMS 114 (人肺癌细胞) (STR鉴定正确) |
| 别称 | DMS-114; DMS114; Darmouth Medical School 114 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
Waymouth's MB 752/1+10%FBS＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
| 年龄（性别） | 男性；68岁 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 3.8 days (PubMed=6266631) |
| 致瘤性 | Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| 抗原表达情况 | Leu 7 +; My23 +; CD11b + |
| 基因表达情况 | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
| 保藏机构 | ATCC; CRL-2066 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11 D2S1338 17 D3S1358 14,17 D5S818 12 D7S820 10,11 D8S1179 12,13 D13S317 13 D16S539 12 D18S51 18 D19S433 13 D21S11 29 FGA 21 PentaD 11,13 PentaE 7,13 TH01 8,9.3 TPOX 8 vWA 16,17 D6S1043 12,17 D12S391 18 D2S441 10 -
STR鉴定图
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