产品概述
产品信息
| 名称 | Ba/F3 (小鼠原B细胞) (STR鉴定正确) |
| 别称 | BA/F3; BaF3; BAF3; Baf3 |
| 种属 | 小鼠 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 圆形 |
| 冻存条件 | 冻存液：90%FBS+10%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
RPMI-1640+10ng/mL mouse IL-3+10%FBS+1%P/S
培养条件:
气相：空气，95%；CO₂，5%, 温度：37℃
|
| 推荐传代比例 | 3×10^5-1×10^6个/mL |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | IL-3 dependent murine pro B cell line; an original publication describing establishment of BA/F3 does not exist; probably a subclone of a first described Ea3 cell line. Performing May-Gruenwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic background, however cells were apparently not derived from BALB/c, but from C3H mouse. |
| 组织来源 | 骨髓 |
| 细胞类型 | 因子依赖细胞系 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~20 hours |
| 保藏机构 | DSMZ; ACC-300 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15,17 X-1 26,27 1-2 16 7-1 26 8-1 16 1-1 10 19-2 11,12 15-3 24.3 12-1 16 6-4 18,19 4-2 19.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 16 -
STR鉴定图
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